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Toward Secure Digital Microfluidic Biochips: Optical and Hash-based Runtime Safeguards

  • New York University
  • New York University Abu Dhabi
  • Indian Institute of Technology Guwahati
  • Arizona State University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Digital microfluidic biochips (DMFBs) integrate micro-fabricated electrode arrays with control software to manipulate droplets in the nanoliter to sub-microliter range for point-of-care diagnostics and biochemical assays. However, their tight hardware-software co-design is vulnerable to stealthy attacks-nanometer-scale tampering of electrode or dielectric thickness, plasma-induced erosion of hydrophobic coatings, and firmware-hidden high-voltage pulses causing dielectric breakdown. We present a layered defense using a spectral-domain optical coherence tomography (SD-OCT) system for non-destructive, micron-resolution depth scans that detect structural anomalies via interface-peak metrics and light backscattering attenuation profiles, alongside a lightweight hash-based attestation protocol to guarantee firmware integrity. Experiments applying OCT to a commercial DMFB and characterizing PDMS dielectrics across 10:1-50:1 curing ratios validated robust detection of microstructural anomalies and material deviations, while hash-based attestation secured firmware integrity-together enabling comprehensive, inline quality control for tamper-resilient DMFBs.

Original languageEnglish
Title of host publicationProceedings - 21st IEEE Biomedical Circuits and Systems, BioCAS 2025
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages457-461
Number of pages5
ISBN (Electronic)9798331573362
DOIs
StatePublished - 2025
Event21st IEEE Biomedical Circuits and Systems, BioCAS 2025 - Abu Dhabi, United Arab Emirates
Duration: 16 Oct 202518 Oct 2025

Publication series

NameProceedings - 21st IEEE Biomedical Circuits and Systems, BioCAS 2025

Conference

Conference21st IEEE Biomedical Circuits and Systems, BioCAS 2025
Country/TerritoryUnited Arab Emirates
CityAbu Dhabi
Period16/10/2518/10/25

Keywords

  • cyber-physical security
  • Digital microfluidic biochips
  • hash-based attestation
  • optical coherence tomography
  • PDMS curing ratio

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