Signature molecular changes in the skeletal muscle of hindlimb unloaded mice

Muhammad Azeem; Rizwan Qaisar; Asima Karim; Anu Ranade; Adel Elmoselhi

doi:10.1016/j.bbrep.2021.100930

Signature molecular changes in the skeletal muscle of hindlimb unloaded mice

University of Sharjah

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Hind-limb unloaded (HU) mouse is a well-recognized model of muscle atrophy; however, the molecular changes in the skeletal muscle during unloading are poorly characterized. We have used Raman spectroscopy to evaluate the structure and behavior of signature molecules involved in regulating muscle structural and functional health. The Raman spectroscopic analysis of gastrocnemius muscles was compared between 16-18 weeks old HU c57Bl/6J mice and ground-based controls. The spectra showed that the signals for asparagine and glutamine were reduced in HU mice, possibly indicating increased catabolism. The peaks for hydroxyproline and proline were split, pointing towards molecular breakdown and reduced tendon repair. We also report a consistently increased intensity in> 1300 cm^-1 range in the Raman spectra along with a shift towards higher frequencies in the HU mice, indicating activation of sarcoplasmic reticulum (SR) stress during HU.

Original language	English
Article number	100930
Journal	Biochemistry and Biophysics Reports
Volume	25
DOIs	https://doi.org/10.1016/j.bbrep.2021.100930
State	Published - Mar 2021

Keywords

Molecular changes
Raman spectroscopy
SR stress
Skeletal muscle

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10.1016/j.bbrep.2021.100930

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title = "Signature molecular changes in the skeletal muscle of hindlimb unloaded mice",

abstract = "Hind-limb unloaded (HU) mouse is a well-recognized model of muscle atrophy; however, the molecular changes in the skeletal muscle during unloading are poorly characterized. We have used Raman spectroscopy to evaluate the structure and behavior of signature molecules involved in regulating muscle structural and functional health. The Raman spectroscopic analysis of gastrocnemius muscles was compared between 16-18 weeks old HU c57Bl/6J mice and ground-based controls. The spectra showed that the signals for asparagine and glutamine were reduced in HU mice, possibly indicating increased catabolism. The peaks for hydroxyproline and proline were split, pointing towards molecular breakdown and reduced tendon repair. We also report a consistently increased intensity in> 1300 cm-1 range in the Raman spectra along with a shift towards higher frequencies in the HU mice, indicating activation of sarcoplasmic reticulum (SR) stress during HU.",

keywords = "Molecular changes, Raman spectroscopy, SR stress, Skeletal muscle",

author = "Muhammad Azeem and Rizwan Qaisar and Asima Karim and Anu Ranade and Adel Elmoselhi",

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language = "English",

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T1 - Signature molecular changes in the skeletal muscle of hindlimb unloaded mice

AU - Azeem, Muhammad

AU - Qaisar, Rizwan

AU - Karim, Asima

AU - Ranade, Anu

AU - Elmoselhi, Adel

PY - 2021/3

Y1 - 2021/3

N2 - Hind-limb unloaded (HU) mouse is a well-recognized model of muscle atrophy; however, the molecular changes in the skeletal muscle during unloading are poorly characterized. We have used Raman spectroscopy to evaluate the structure and behavior of signature molecules involved in regulating muscle structural and functional health. The Raman spectroscopic analysis of gastrocnemius muscles was compared between 16-18 weeks old HU c57Bl/6J mice and ground-based controls. The spectra showed that the signals for asparagine and glutamine were reduced in HU mice, possibly indicating increased catabolism. The peaks for hydroxyproline and proline were split, pointing towards molecular breakdown and reduced tendon repair. We also report a consistently increased intensity in> 1300 cm-1 range in the Raman spectra along with a shift towards higher frequencies in the HU mice, indicating activation of sarcoplasmic reticulum (SR) stress during HU.

AB - Hind-limb unloaded (HU) mouse is a well-recognized model of muscle atrophy; however, the molecular changes in the skeletal muscle during unloading are poorly characterized. We have used Raman spectroscopy to evaluate the structure and behavior of signature molecules involved in regulating muscle structural and functional health. The Raman spectroscopic analysis of gastrocnemius muscles was compared between 16-18 weeks old HU c57Bl/6J mice and ground-based controls. The spectra showed that the signals for asparagine and glutamine were reduced in HU mice, possibly indicating increased catabolism. The peaks for hydroxyproline and proline were split, pointing towards molecular breakdown and reduced tendon repair. We also report a consistently increased intensity in> 1300 cm-1 range in the Raman spectra along with a shift towards higher frequencies in the HU mice, indicating activation of sarcoplasmic reticulum (SR) stress during HU.

KW - Molecular changes

KW - Raman spectroscopy

KW - SR stress

KW - Skeletal muscle

UR - https://www.scopus.com/pages/publications/85099956849

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DO - 10.1016/j.bbrep.2021.100930

M3 - Article

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VL - 25

JO - Biochemistry and Biophysics Reports

JF - Biochemistry and Biophysics Reports

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ER -

Signature molecular changes in the skeletal muscle of hindlimb unloaded mice

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Keywords

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