Abstract
Background: Carbamazepine (CBZ) is widely used as an anti-epileptic drug. Vitamin B12 has been shown to protect against DNA damage caused by several mutagenic agents. Objective: This study aimed to investigate the effect of vitamin B12 on CBZ-induced genotoxicity in cultured human lymphocytes. Methods: Sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs) genotoxic assays were utilized to achieve the study objective. Results: The results showed significantly higher frequencies of CAs and SCEs in the CBZ-treated cultures (12 μg/mL) compared to the control group (P<0.01). The genotoxic effects of CBZ were reduced by pre-treatment of cultures with vitamin B12 (13.5μg/ml, P<0.05). Neither CBZ nor vitamin B-12 showed any effects on mitotic and proliferative indices. Conclusion: CBZ is genotoxic to lymphocyte cells, and this genotoxicity can be reduced by vitamin B12.
| Original language | English |
|---|---|
| Pages (from-to) | 228-233 |
| Number of pages | 6 |
| Journal | Current Molecular Pharmacology |
| Volume | 16 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2023 |
Keywords
- Carbamazepine
- DNA damage
- chromosomal aberrations
- genotoxicity
- sister chromatid exchanges
- vitamin B12
- white lymphocytes
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